Ashby’s Glucose Agar

Principle Ashby’s glucose agar is described by Subba Rao (1977) for the isolation of a non-symbiotic nitrogen-fixing bacteria, Azotobacter. Ashby’s media is composed of glucose, dipotassium phosphate, magnesium sulfate, sodium chloride, potassium sulfate, and calcium carbonate. Glucose provides a carbon source and atmospheric nitrogen serves nitrogen source. Dipotassium Phosphate is a buffering agent. The other salts provide various essential ions required for promoting the growth of Azotobacter. The presences of calcium carbonate stimulate growth and increase the availability of phosphorus, sulfur, and magnesium to the organisms. Use: Recommended for isolation of Glucose fermenting Azotobacter from soil samples. PARAMETERS STANDARD VALUES PHYSICAL PARAMETERS: Dehydrated Powder A fine, free-flowing, hygroscopic powder. Color Off-white colored powder. Solution Solubility Soluble in Distilled / de-ionized water at 50˚ C Color Milky white colored solution with stealing at the bottom on standing pH 7.4 ±0.2 at 25 ˚C (40.70 g/ l) Agar Plates Solidification 40 ˚C Color Milky white colored opalescent gel with slight precipitate. ,Intend use : • 18% GST extra on Product • Packaging, Handling & Transport charges (with GST) extra as applicable. • Delivery period : 3-4 weeks or earlier as per availability.